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Payne, Gregory F.

Payne, Gregory F.

Research Interests 

Nanobiotechnology, biofabrication (construction using biological materials and mechanisms), stimuli-responsive biopolymers (e.g., chitosan and alginate), enzymes (e.g., tyrosinase and microbial transglutaminase), electroaddressing, renewable resources.
 

Education 

Ph.D., The University of Michigan, 1984

Current Research Projects 

Biology is expert in creating functional nanoscale components (e.g., proteins) and assembling them over a hierarchy of length scales to create functional structures (e.g., organs). Our lab aims to enlist biology’s materials, mechanisms and lessons to fabricate high-performance soft matter that is cheap, safe and sustainable. In particular, we focus on building structure/function using stimuli-responsive biological polymers (especially polysaccharides), enzymes (especially tyrosinase and transglutaminase), and redox-active phenolics. Currently we are collaborating with several groups from around the world in three primary areas of research.

Biofabrication of the Bio-device Interface

The last century witnessed spectacular advances in both microelectronics and biotechnology yet there was little synergy between the two. A challenge to their integration is that biological and electronic systems are constructed using divergent fabrication paradigms. Biology fabricates bottom-up with labile components while microelectronic devices are fabricated top-down using methods that are “bio-incompatible”. Biofabrication – especially the use of biological materials and mechanisms for construction – offers the opportunity to span these fabrication paradigms by providing convergent approaches for building the bio-device interface.

Figure 1 (below) illustrates our vision for biofabricating the bio-device interface. Device-imposed stimuli provide the cues to initiate assembly and control spatiotemporal localization. Integral to this vision are stimuli-responsive materials that recognize the device-imposed cues and respond by undergoing a sol-gel transition to deposit as stable hydrogel films. Importantly, hydrogel electrodeposition provides a mechanism to trigger self-assembly over a hierarchy of length scales and yields a water-rich microenvironment that is generally compatible with labile biological systems. A second mechanism for hierarchical assembly employs enzymes that covalently conjugate macromolecules (e.g., proteins) to the self-assembled matrix. Through various collaborations, we are expanding and applying these biofabrication tools to biofunctionalize microfluidic lab-on-a-chip devices.
 

biofabrication
Fig. 1. Biofabrication to build the bio-device interface.
Biofabrication 2022002 (2010).

 

Biofabricated Redox-capacitor to Establish Bio-device "Connectivity"

Biology and electronics each possess incredible signaling processing capabilities – but they use different signaling modalities. Biology signals using ions and molecules while electronic devices use electrons to process information. Oxidation-reduction (i.e., redox) reactions provide the bridge to connect biological and electronic communication.

We recently fabricated a bio-based redox-capacitor by depositing a thin film of the polysaccharide chitosan and then modifying this film with catecholic moieties. Catechols/quinones are common redox-couples in biology and we observed that these moieties confer redox-activity to the chitosan film. Importantly, the catecholic matrix is redox-active (it can exchange electrons with appropriate mediators) but is non-conducting (it cannot exchange electrons directly with the underlying electrode). These physicochemical properties allow the catecholic matrix to be switched between two stable states (oxidized and reduced) and also allows the matrix to gate, amplify and partially-rectify mediator currents. Also, this catecholic matrix can exchange electrons with bio-relevant oxidants and reductants (e.g., NADH and ascorbate). As illustrated in Figure 2 (below), this catecholic redox-capacitor can interconnect biological and electrical inputs/outputs for information processing.

catecholic matrix
Figure 2. The catecholic matrix (QH2/Q) can exchange electrons with the redox-active metabolites pyocyanin (PYO) and acetosyringone (AS) that can access cellular redox-state. In addition, these metabolites can shuttle electrons between the electrode and the matrix. These properties allow cyclic potential inputs to be imposed to generate steady output currents that possess information of biological/environmental redox activities.

 

Biopolymeric Materials as High-performance Alternatives

Traditionally, biopolymeric materials have been viewed as bio-based alternatives to synthetic polymers. The commonly-stated advantages of bio-based polymers are their sustainable source and their environmental-friendliness (i.e., biodegradability). However, biopolymers often possess functionality that allows them to compete in terms of performance. In fact, the high-performance capabilities of proteins are well-established: proteins are expressed with controlled sequence, size and shape, and they possess unparalleled capabilities for molecular recognition and catalysis.

Our lab focuses on polysaccharides and phenolics. Polysaccharides are routinely used in food and consumer applications because they possess valuable functional properties (e.g., viscosifying and gelling properties). Often these properties change dramatically in response to small changes in environmental conditions (e.g., in pH, temperature and solution composition). Phenolic-based materials (e.g., lignin and melanin) are more abundant in nature than either proteins or nucleic acids, yet they are seldom studied. Often phenolics possess optical and redox properties that confer protective functions. Over the long term, our lab’s biofabrication research should promote the broader use of polysaccharides and phenolics for food, cosmetic, pharmaceutical, biotechnology and medical applications where performance and biological compatibility are essential.

Honors and Awards 

  • Guest Professor, Wuhan University, China

Selected Publications 

Coupling Electrodeposition with Layer-by-Layer Assembly to Address Proteins within Microfluidic Channels. Advanced Materials. 23: 5817-5821 (2011).

Electroaddressing Functionalized Polysaccharides as Model Biofilms for Interrogating Cell Signaling. Advanced Functional Materials. 22: 519-528 (2012).

Electrodeposition of a Biopolymeric Hydrogel: Potential for One-step Protein Electroaddressing. Biomacromolecules. 13: 1181-1189 (2012).

Biofabrication of Stratified Biofilm Mimics for Observation and Control of Bacterial Signaling. Biomaterials. 33: 5136-5143 (2012).

Biofabricating Multi-functional Soft Matter with Enzymes and Stimuli-Responsive Materials. Advanced Functional Materials. In Press (2012).

Redox Cycling and H2O2-Generation by Fabricated Catecholic Films in the Absence of Enzymes. Biomacromolecules, 12: 880-888 (2011).

Biomimetic Fabrication of Information-rich Phenolic Chitosan Films. Soft Matter, 7: 9601-9615 (2011).

Redox Capacitor to Establish Bio-device Redox-Connectivity. Advanced Functional Materials. 22: 1409-1416 (2012).

Payne, G.F., P.B. Smith (Eds). “Renewable and Sustainable Polymers.” American Chemical Society Press. Washington, DC (2011).

Related News 

BIOE Capstone 2017: Projects Focus on Pediatric Care, Physical Therapy, Pulmonary Tumors, and More
BIOE hosted it's largest-ever Capstone Design competition on May 10th, 2017. May 17, 2017

UMD Researchers Make Strides in Schizophrenia Diagnosis Research
Blood test could help doctors more quickly diagnose schizophrenia and other disorders. February 9, 2017

Bentley, Payne Featured in The Conversation
Distinguished University Professor William Bentley publishes piece on using electricity to control gene expression. January 18, 2017

BIOE, IBBR Researchers Develop Electrogenetic Device for Activating Gene Expression via Electrodes
System holds promise for study of biological systems, biosensors and bio-hybrid devices. January 17, 2017

Payne Appointed Guest Professor at South China University of Technology
Bioengineering professor begins three-year guest professorship with multidisciplinary university. January 10, 2017

BioE Researchers Develop Nano-guided Cell Networks as Conveyors of Molecular Communication
BioE Professor and Founding Chair William Bentley’s research group publishes Nature Communications paper. November 12, 2015

Gray Named 2014 Fischell Fellow
BioE graduate student working to develop blood-brain barrier-on-a-chip. November 14, 2014

UMD Researchers Bridge Gap between Microelectronics, Biological Systems
“Electronic modulation of biochemical signal generation” published in Nature Nanotechnology July 28, 2014

BioE Hosts First Luojia International Biomedical Conference
Event fosters collaborations between UMD and Wuhan University. October 3, 2013

Science Gets Bentley and Payne's Perspective on Self-Assembly
BioE/IBBR professors invited to share views on new materials for biomedical applications. July 11, 2013

Payne Honored with Guest Professorship
Wuhan University awards three-year appointment. March 29, 2013

Preventing Costly, Life-Threatening Catheter Infections
Deutsch Foundation-sponsored Clark School research offers multi-pronged attack on major medical problem. January 18, 2012

Bacteria Programmed to Re-Create UMD Logo
Feat part of larger body of Clark School research into preventing infections. December 13, 2011

Bentley, Payne Receive USM Regents' Awards for Nano-, Biodevice Work
Professors recognized for exemplary scholarship and research. March 14, 2011

Payne, Shapiro Join BioE Faculty
Former affiliates focus on miniaturized systems, biofabrication, nanobiotechnology. May 21, 2010

Bentley Group Research Selected for Highlights in Chemical Technology
Paper covers monitoring and controlling bacterial communication. March 29, 2010

Rubloff Group Research Selected for Highlights in Chemical Technology.
Paper covers advances in lab-on-a-chip technology. November 20, 2009

Berlin Takes 2nd in Dean's M.S. Thesis Competition
New award honors outstanding research, scholarship. May 19, 2009

Payne, Bentley Research Wins TEDCO Funding
Award supports efforts to commercialize chitosan-based biochip. January 6, 2009

Kofinas on Food Safety at USDA Meeeting
Presents work on food pathogen detection using intelligent packaging. September 22, 2008

Quorum Sensing Research Wins $2.5M DoD Contract
Bioeningeering/UMBI collaborate on biological threat detection. June 19, 2008

WJZ Covers New Chitosan Blood Clotting Product
Dowling, Raghavan, Payne featured in TV segment. May 21, 2008

Advance in Creating in Vitro Programmable Biological Microfactories
Research team includes Bentley, Ghossi, Payne, Rubloff. March 27, 2008

Nightly Business Report Segment Now Online
BioE faculty discuss nanotech’s role in food safety. November 14, 2007

$2M NSF Grant for "Bacterial Communication"
Cellular and biomolecular engineering research to focus on biofunctionalized devices. August 28, 2007

Kofinas, Payne Interviewed for PBS’ Nightly Business Report
Professors explain nanotechnology's potential role in food safety. July 26, 2007

Crab Nano-Sensor Detects Dangerous Substances
A material found in crab shells is part of a new sensor system. July 26, 2006