Professor and Chair William Bentley

Chair
Robert E. Fischell Distinguished Professor
Joint appointment with IBBR.
Ph.D., University of Colorado at Boulder, 1989

Room 2330 Jeong H. Kim Engineering Building
Email: bentley@umd.edu
Phone: (301) 405-4321 
Fax: (301) 405-9953

For more information please visit:
Message from the Chair »
The Biochip Collaborative »
Group Page: Molecular & Metabolic Engineering Laboratories »

Background Information

William E. Bentley is the Robert E. Fischell Distinguished Professor of Engineering and founding Chair of the Fischell Department of Bioengineering. He is also appointed in the Department of Chemical and Biomolecular Engineering at the University of Maryland, College Park and the Institute for Bioscience and Biotechnology Research. Dr. Bentley received his undergraduate (BS, '82) and Master of Engineering degrees ('83) from Cornell University and his Ph.D. ('89) from the University of Colorado, Boulder, all in chemical engineering. At Maryland since 1989, Dr. Bentley has focused his research on the development of molecular tools that facilitate the expression of biologically active proteins, having authored over 200 related archival publications. Recent interests are on deciphering and manipulating signal transduction pathways, including those of bacterial communication networks, for altering cell phenotype. He has served on advisory committees and panels for the NIH, NSF, DOD, DOE, USDA, and several state agencies. He has mentored over 25 Ph.D.s, some of whom are academics at: Cornell (x2), University of Colorado, Clemson, University of Connecticut, Tufts, Postech (Korea), and Tsing-Hua (Taiwan). He co-founded a protein manufacturing company, Chesapeake PERL, based on insect larvae as mini bioreactors. Dr. Bentley is a Fellow of the AAAS and AIMBE and is an elected member of the American Academy of Microbiology.

Research Interests

Biomolecular and metabolic engineering, cell-to-cell communication, heterologous protein expression, device/bio interfaces.

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Honors and Awards

• Robert E. Fischell Distinguished Professor
• Herbert Rabin Distinguished Professor (former)
• University System of Maryland (USM) Regents' Faculty Award for Research/Scholarship/Creative Activity (2011)
• Vice President At-Large, American Institute for Medical and Biological Engineering, 2011-2014
• U.S. EPA, Scientific and Technological Achievement Award, Level II (2010)
• Member, Advisory Council, Dept. of Chemical and Biomolecular Engineering, Cornell University (2008-2011)
• Member, Departmental Advisory Committee, Department of Biomedical Engineering, Cornell University (2009-2012)
• Member, International Scientific Advisory Board, Austrian Center of Industrial Biotechnology (ACIB) (2009)
• Elected Member, Managing Board, Society for Biological Engineers (2008)
• Fellow, American Academy of Microbiology (AAM)
• Fellow, American Association for the Advancement of Science
• Fellow, American Institute for Medical and Biological Engineering
• Schering-Plough Young Investigator Award (1996)
• Dow Outstanding New Faculty Award from the American Society for Engineering Education (1995)
• Society of Industrial Microbiology Outstanding Achievement in Engineering Sciences Award
• Washington Academy of Sciences Allen C. Davis Medal
• Maryland's Outstanding Young Engineer, Maryland Science Center

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Selected Recent Refereed Publications

1. Cheng, Y., Tsao, C.-Y., Wu, H.-C., Luo, X.L., Terrell, J.L., Betz, J., Payne, G.F., Bentley, W.E., and G.W. Rubloff (2012) Electroaddressing Functionalized Polysaccharides as Model Biofilms for Interrogating Cell Signaling, Advanced Functional Materials, 22(3):519-528.

Terrell, J.L., Gordonov, T., Cheng, Y., Wu, H.C., Sampey, D., Luo, X., Tsao, C.Y., Ghodssi, R., Rubloff, G.W., Payne, G.F., and W.E. Bentley (2011) Integrated Biofabrication for Electro-Addressed In-Film Bioprocessing, Biotechnology Journal, (Epub, PMID 22213675).

Carter, K.K., Valdes, J.J., and W.E. Bentley (2011) Pathway Engineering via Quorum Sensing and sRNA Riboregulators – Interconnected Networks and Controllers, Metabolic Engineering, (Epub, PMID22155614).

Luo, X.L., Buckhout-White, S., Bentley, W.E., and G.W. Rubloff (2011) Biofabrication of Chitosan-Silver Composite SERS Substrates Enabling Quantification of Adenine by a Spectroscopic Shift, Biofabrication, 3(3):034108.

Cheng, Y., Luo, X., Tsao, C.-Y., Wu, H.C., Betz, J., Payne, G.F., Bentley, W.E., and G.W. Rubloff (2011) Biocompatible multi-address 3D Cell Assembly in Microfluidic Devices Using Spatially Programmable Gel Formation, Lab on a Chip, 11(14):2316-2318.

Kamaraju, K., Smith, J., Wang, J., Roy, V., Sintim, H.O., Bentley, W.E., and S. Sukharev (2011) Effects on Membrane Lateral Pressure Suggest Permeation Mechanisms for Bacterial Quorum Sensing Molecules, Biochemistry, 50(32):6983-93.

Wang, Y., Liu, Y., Cheng, Y., Kim, E., Rubloff, G.W., Bentley, W.E., and G.F. Payne (2011) Coupling Electrodeposition with Layer-by-Layer Assembly to Address Proteins within Microfluidic Channels, Advanced Materials, 23(8):5817-5821

Hooshangi, S., and W.E. Bentley (2011) LsrR Quorum Sensing “Switch” is Revealed by a Bottom-up Approach, PLoS Computational Biology, 7(9):e1002172.

Dykstra, P.H., Roy, V., Byrd, C., Bentley, W.E., and R. Ghodssi (2011) Microfluidic Electrochemical Sensor Array for Characterizing Protein Interactions with Various Functionalized Surfaces, Analytical Chemistry, 83(15):5920-5927.Dykstra, P.H., Roy, V., Byrd, C., Bentley, W.E., and R. Ghodssi (2011) Microfluidic Electrochemical Sensor Array for Characterizing Protein Interactions with Various Functionalized Surfaces, Analytical Chemistry, 83(15):5920-5927.

Roy, V., Adams, B.L., and W.E. Bentley (2011) Developing Next Generation Antimicrobials by Intercepting AI-2 Mediated Quorum Sensing, Enzyme and Microbial Technology, 49(2): 113-123.

Liu, Y., Yi, C., Wu, H.C., Kim, E., Ulijn, R.V., Rubloff, G.W., Bentley, W.E., and G.F. Payne (2011) Electroaddressing Agarose Using Fmoc-Phenylalanine as a Temporary Scaffold, Langmuir, 27(12):7380-7384.

Liu, Y., Kim, E., Ulijn, R.V., Bentley, W.E., and G.F. Payne (2011) Reversible Electroaddressing of Self-assembling Amino-Acid Conjugates, Advanced Functional Materials, 21(9):1575-1580.

Meyer, M.T., Roy, V., Bentley, W.E., and R. Ghodssi (2011) Development and Validation of a Microfluidic Reactor for Biofilm Monitoring via Optical Methods, Journal of Micromechanics and Microengineering, 21(5) (#054023).

Tsao, C.Y., Wang, L., Hashimoto, Y., Yi, H., March, J.C., Delisa, M.P., Wood, T.K., Valdes, J.J., and W.E. Bentley (2011) LuxS Co-expression Enhances Yield of Recombinant Proteins in E. coli in part through Post-transciptional control of GroEL. Applied and Environmental Microbiology, 77(6):2141-2152.

Liu, Y., Shi, X.W., Kim, E., Robinson, L.M., Nye, C.K., Ghodssi, R., Rubloff, G.W., Bentley, W.E., and G.F. Payne (2011) Chitosan to Electroaddress Biological Components in Lab-on-a-Chip Devices, Carbohydrate Polymers, 84(2):704-708.

Wu, L.Q., Bentley, W.E., and G.F. Payne (2011) Biofabrication with biopolymers and enzymes: Potential for constructing scaffolds from soft matter, International Journal of Artificial Organs, (E-Pub).

Kim, E., Liu, Y., Baker, C.J., Owens, R., Xiao, S., Bentley, W.E., and G.F. Payne (2011) Redox-cycling and H(2)O(2) Generation by Fabricated Catecholic Films in the Absence of Enzymes, Biomacromolecules, 12(4):880-888.

Gray, K.M., Kim, E., Wu. L-Q., Liu, Y., Bentley, W.E., and G.F. Payne (2011) Biomimetic Fabrication of Information-Rich Phenolic-Chitosan Films, Soft Matter, 7(20):9601-9615.

Cheng, Y., Luo, X., Betz, J., Payne, G.F., Bentley, W.E., and G.W. Rubloff (2011) Mechanism of Anodic Electrodeposition of Calcium Alginate, Soft Matter, 7(12), 5677-5684.

Nde, C.W., Toghrol, F., Jang, H.J., and W.E. Bentley (2010) Toxicogenomic Response of Mycobacterium bovis BCG to Peracetic Acid and a Comparative Analysis of the M. Bovis BSG Response to Three Oxidative Disinfectants, Applied Microbiology and Biotechnology, 90(1):277-304.

Cheng, Y., Luo, X.L., Betz, J., Buckhout-White, S., Bekdash, O., Payne, G.F., Bentley, W.E., and G.W. Rubloff (2010) In situ Quantitative Visualization and Characterization of Chitosan Electrodeposition with Paired Sidewall Electrodes, Soft Matter, 6(14): 3177-3183.

Yung, C.W., Bentley, W.E., and T.A. Barbari (2010) Diffusion of Interleukin-2 from Cells Overlaid with Cytocompatible Enzyme-Crosslinked Gelatin Hydrogels, Journal of Biomedical Materials Research Part A, 95(1):25-32.

Koev, S.T., Dykstra, P.H., Luo, X., Rubloff, G.W., Bentley, W.E., Payne, G.F., and R. Ghodssi (2010) Chitosan: an Integrative Biomaterial for Lab-on-a-chip Devices, Lab on a Chip, 10(22):3026-3042.

Hebert, C., Gupta, A., Tsao, C-Y., Valdes, J.J., and W.E. Bentley (2010) Biological Nanofactories target and Activate Epithelial Cell Surfaces for Modulating Bacterial Quroum Sensing and Interspecies Signaling, ACS Nano, 4(11): 6923-6931.

Bauer, R.I., Luo, X., and W.E. Bentley (2010) Chips & Tips: Customizable PDMS Chamber for Cell Imaging, Lab on a Chip. (peer-reviewed on-line supplement to journal).

Roy, V., Smith, J.A., Wang, J., Stewart, J.E., Bentley, W.E., and H.O. Sintim (2010) Synthetic Analogs Tailor AI-2 Signaling Across Bacterial Species, Journal of the American Chemical Society, 132(32):11141-11150.

Kim, E., Liu, Y., Shi, X.W., Yang, X., Bentley, W.E., and G.F. Payne (2010) Biomimetic Approach to Confer Redox Activity to Thin Chitosan Films, Advanced Functional Materials, 20(16): 2683-2694.

Liu, Y., Kim, E., Ghodssi, R., Rubloff, G.W., Culver, J.N., Bentley, W.E., and G.F. Payne (2010) Biofabrication to Build the Biology-Device Interface, Biofabrication, 2(2):220.

Yang, X.H., Kim, E., Liu, T., Shi, X.W., Rubloff, G.W., Ghodssi, R., Bentley, W.E., and G.F. Payne (2010) In-film Bioprocessing and Immunoanalysis with Electroaddressable Stimuli-Responsive Polysaccharides, Advanced Functional Materials, 20(10):1645-1652.

Koev, S.T., Bentley, W.E., Ghodssi, R (2010) Interferometric readout of multiple cantilever sensors in liquid samples, Sensors and Actuators B – Chemical, 146(1):245-252.

Roy, V., Fernandes, R., Tsao, C.Y., and W.E. Bentley (2010) Cross Species Quorum Quenching Using A Native AI-2 Processing Enzyme, ACS Chemical Biology, 5(2):223-232.

Fernandes R., Luo, X., Tsao, C.-Y., Payne, G.F., Ghodssi, R., Rubloff, G.W., and W.E. Bentley (2010) Biological Nanofactories Facilitate Spatially Selective Capture and Manipulation of Quorum-Sensing Bacteria in a BioMEMS Device, Lab on a Chip, 10(9):1128-1134

Fernandes, R., Roy, V., Wu, H.C., and W.E. Bentley (2010) Engineered biological nanofactories trigger quorum sensing response in targeted bacteria, Nature Nanotechnology, 5(3)213-217.

Tsao, C.Y., Hooshangi, S., Wu, H.C., Valdes, J.J., and W.E. Bentley (2010) Autonomous Induction of Recombinant Proteins by Minimally Rewiring Native Quorum Sensing Regulon of E. coli, Metabolic Engineering, 12(3):291-297.

Luo X., Berlin, D.L., Betz, J., Payne, G.F., Bentley, W.E., and G.W. Rubloff (2010) In situ generation of pH gradients in microfluidic devices for biofabrication of freestanding, semi-permeable chitosan membranes, Lab on a Chip, 10(1): 59-65.

Yung, C.W., Bentley, W.E., and T.A. Barbari (2010) Diffusion of interleukin-2 from cells overlaid with cytocompatible enzyme-crosslinked gelatin hydrogels, Journal of Biomedical Materials Research: Part A (in press).

Roy, V., Fernandes, R., Tsao, C.Y., and W.E. Bentley (2010) Cross Species Quorum Quenching Using A Native AI-2 Processing Enzyme, ACS Chemical Biology, 5(2):223-232.

Tsao, C.Y., Hooshangi, S., Wu, H.C., Valdes, J.J., and W.E. Bentley (2010) Autonomous Induction of Recombinant Proteins by Minimally Rewiring Native Quorum Sensing Regulon of E. coli, Metabolic Engineering, 12(3):291-297.

Fernandes, R., Roy, V., Wu, H.C., and W.E. Bentley (2010) Engineered biological nanofactories trigger quorum sensing response in targeted bacteria, Nature Nanotechnology, 5(3)213-217.

Fernandes R., Luo, X., Tsao, C.-Y., Payne, G.F., Ghodssi, R., Rubloff, G.W., and W.E. Bentley (2010) Biological Nanofactories Facilitate Spatially Selective Capture and Manipulation of Quorum-Sensing Bacteria in a BioMEMS Device, Lab on a Chip, 10(9):1128-1134.

Roy, V., Fernandes, R., Tsao, C.Y., and W.E. Bentley (2010) Cross Species Quorum Quenching Using A Native AI-2 Processing Enzyme, ACS Chemical Biology, 5(2):223-232.

Koev, S.T., Bentley, W.E., Ghodssi, R (2010) Interferometric readout of multiple cantilever sensors in liquid samples, Sensors and Actuators B–Chemical, 146(1):245-252.

Yang, X.H., Kim, E., Liu, T., Shi, X.W., Rubloff, G.W., Ghodssi, R., Bentley, W.E., and G.F. Payne (2010) In-film Bioprocessing and Immunoanalysis with Electroaddressable Stimuli-Responsive Polysaccharides, Advanced Functional Materials, 20(10):1645-1652.

Liu, Y., Kim, E., Ghodssi, R., Rubloff, G.W., Culver, J.N., Bentley, W.E., and G.F. Payne (2010) Biofabrication to Build the Biology-Device Interface, Biofabrication, 2(2):220.

Kim, E., Liu, Y., Shi, X.W., Yang, X., Bentley, W.E., and G.F. Payne (2010) Biomimetic Approach to Confer Redox Activity to Thin Chitosan Films, Advanced Functional Materials, (in press).

Roy, V., Smith, J.A., Wang, J., Stewart, J.E., Bentley, W.E., and H.O. Sintim (2010) Synthetic Analogs Tailor AI-2 Signaling Across Bacterial Species, Journal of the American Chemical Society, (online).

Bauer, R.I., Luo, X., and W.E. Bentley (2010) Chips & Tips: Customizable PDMS Chamber for Cell Imaging, Lab on a Chip, (in press).

Hebert, C., Gupta, A., Tsao, C-Y., Valdes, J.J., and W.E. Bentley (2010) Biological Nanofactories target and Activate Epithelial Cell Surfaces for Modulating Bacterial Quroum Sensing and Interspecies Signaling, ACS Nano, (e pub).

Lee, J., Bansal, T., Jayaraman, A., Bentley, W.E., and T.K. Wood (2007) Enterohemorrhagic Escherichia coli Biofilms are Inhibited by 7-Hydroxyindole and Stimulated by Isatin, Applied and Environmental Microbiology.

Yung, C.W., Tullman, J.A., Payne, G.F., Bentley, W.E., and T.A. Barbari (2007) Transglutaminase Crosslinked Gelatin as a Tissue Engineering Scaffold, Journal of Biomedical Materials Research A, (Epub ahead of print).

Li, J., Attila, C., Wang, L., Wood, T.K., Valdes, J.J., and W.E. Bentley (2007) Quorum Sensing in E. coli is Signaled by AI-2/LsrR: Effects on sRNA and Biofilm Architecture, Journal of Bacteriology, 73:4100-4109.

Fernandes, R., Tsao, C.Y., Hashimoto, Y., Wang, L., Wood, T.K., Payne, G.F., and W.E. Bentley (2007) Magnetic Nanofactories: Localized Synthesis and Delivery of Quorum-Sensing Signaling Molecule Autoinducer-2 to Bacterial Cell Surfaces, Metabolic Engineering, 9:228-239.

Small, D.A., Chang, W., Bentley, W.E., and F. Toghrol (2007) Toxicogenomic Analysis of Sodium Hypochlorite Antimicrobial Mechanisms in Pseudomonas aeruginosa, Applied Microbiology and Biotechnology, 74:176-185.

Kim, E.J., Kramer, S.F., Hebert, C.G., Valdes, J.J., and W.E. Bentley (2007) Metabolic Engineering of the Baculovirus-Expression System via Inverse "Shotgun" Genomic Analysis & RNA Interference (dsRNA) Increases Product Yield and Cell Longevity, Biotechnology and Bioengineering, 98:645-654.

March, J.C., and W.E. Bentley (2007) RNAi-based Tuning of Cell Cycling in Drosophila S2 cells- effects on Recombinant Protein Yield, Applied Microbiology and Biotechnology, 73: 1128-1135.

Koev, S.T., Powers, M. A. Powers, Yi, H.M., Wu, L-Q., Bentley, W.E., Rubloff, G.W., Payne, G.F., and R. Ghodssi, (2007) Mechano-transduction of DNA Hybridization and Dopamine Oxidation through Electrodeposited Chitosan Network, Lab on a Chip, 7:103-111.

Small, D.A, Chang, W., Toghrol, F., and W.E. Bentley (2007) Comparative Global Transcription Analysis of Sodium Hypochlorite, Peracetic Acid, and Hydrogen Peroxide on Pseudomonas aeruginosa, Applied Microbiology and Biotechnology, 76:1093-1105.

O’Connell, K.P., Kovaleva, E., Campbell, J.H., Anderson, P.E., Brown, S.G., Davis, D.C., Valdes, J.J., Welch, R.W., Bentley, W.E. and N.A. van Beek (2007) Production of a Recombinant Antibody Fragment in Whole Insect Larvae, Molecular Biotechnology, 36:44-51.

Hung, C-W., Holoman, T.R.P., Kofinas, P., and W.E. Bentley (2007) Towards Oriented Assembly of Proteins onto Magnetic Nanoparticles, Biochemical Engineering Journal.

Saini, S.S., Stanford, C., Lee, S.M., Park, J., DeShong, P., Bentley, W.E., and M. Dagenais (2007) Monolayer Detection of Biochemical Agents Using Etched-Core Fiber Bragg Grating Sensors, IEEE Photonics Technology Letters, 19:1341-1343.

Lewandowski, A.T., Yi, H., Luo, X., Payne, G.F., Ghodssi, R., Rubloff, G.W., and W.E. Bentley (2007) Protein Assembly onto Patterned Microfabricated Devices through Enzymatic Activation of Fusion Pro-tag. Biotechnology and Bioengineering.

March, J.C., and W.E. Bentley (2007) Methods for gene silencing with RNAi, Methods in Molecular Biology, 388:424-434.

Hu, Y.C., and W.E. Bentley (2007) Alternative Bioreactor Strategy for Probing Infection and Production, Methods in Molecular Biology, 388:419-426.

Cha, H.J., and W.E. Bentley (2007) Monitoring and Visualization of Baculovirus Infection Using Green Fluorescent Protein Strategy, Methods in Molecular Biology, 388:407-418.

Lewandowski, A.T., Small, D.A., Chen, T., Payne, G.F., and W.E. Bentley (2006) Tyrosine-based "activatable pro-tag": enzyme-catalyzed protein capture and release, Biotechnology and Bioengineering, 93:1207-1215.

Chang, W., Small, D.A., Toghrol, F., and W.E. Bentley (2006) Global Transcriptome Analysis of Staphylococcus aureus Response to Hydrogen Peroxide, Journal of Bacteriology, 188:1648-1659.

Gonzalez Barrios, A.F., Zuo R., Hashimoto, Y., Yang, L., Bentley W.E., and T.K. Wood (2006) Autoinducer 2 Controls Biofilm Formation in Escherichia coli Through a Novel Motility Quorum-sensing Regulator (MqsR, B3022), Journal of Bacteriology, 188:205-316.

Bleckwenn, N. A. and Bentley, W.E. (2006) Systems and Technology Involving Bacteria, In CRC BME Handbook: Tissue Engineering and Artificial Organs (JD Bronzino, ed.) CRC Press, Boca Raton, FL, 2.1-2.10.

Yung, C., Barbari, T.A., and W.E. Bentley (2006) Integrated Non-Invasive System for Quantifying Secreted Human Therapeutic hIL2, Biotechnology and Bioengineering.

Yang, L., Portugal, F.H., and W.E. Bentley (2006) Conditioned Medium from Listeria innocua Stimulate Emergence from a Resting State not in Response to Quorum Sensing Autoinducer AI-2, Biotechnology Progress, 22:387-393.

March, J.C., and W.E. Bentley (2006) Engineering Eukaryotic Signal Transduction with RNAi: Enhancing Drosophila S2 Cell Growth and Recombinant Protein Synthesis via Silencing of TSC1. Biotechnology and Bioengineering.

Park, J.J., Valentine, T.M., Yi, H., Payne, G.F., Bentley, W.E., Ghodssi, R., and Gary W. Rubloff (2006) In situ Biomolecule Assembly in Completely Packaged Microfluidic Devices, Lab on a Chip.

Yung, C.W., Barbari, T.A., and W.E. Bentley (2006) Counteracting Apoptosis and Necrosis with Hypoxia Responsive Expression of Bcl-2D, Metabolic Engineering.

Cresce, A.V., Silverstein, J.S., Bentley, W.E., and P. Kofinas (2006) Nanopatterning of Recombinant Proteins using Block Copolymer Templates, Macromolecules, 39, 5826-5829.

Dalal, N.G., Cha, H.J., Kramer, S.F., Kostov, Y., Rao, G. and W.E. Bentley (2006) Rapid Non-invasive Monitoring of Baculovirus Infection for Insect Larvae Using Green Fluorescent Protein Reporter Under Early-to-Late Promoter and a GFP-Specific Optical Probe, Process Biochemistry, 41:947-950.

Li, J., Wang, L., Hashimoto, Y., Tsao, C-Y., Wood, T.K., Valdes, J.J., Zafiriou, E., and W.E. Bentley (2006) Stochastic Model of E. coli AI-2 Quorum Signal Circuit Reveals Alternative Synthesis Pathways, Nature Molecular Systems Biology.

Wang, L., Hashimoto, Y., Tsao, C.-Y., Valdes, J.J., and W.E. Bentley (2005) cAMP and cAMP Receptor Protein (CRP) Influence Both  Synthesis and Uptake of Extracellular Autoinducer-2 in Escherichia coli, Journal of Bacteriology, 187:2066-76.

Yi, H., Wu, L.Q., Rubloff, G.W., Ghodssi, R., Payne, G.F., and Bentley, W.E., (2005) Signal-Directed Sequential Assembly of Biomolecules on Patterned Surfaces. Langmuir, 2:2104-2107.

Tao, Y., Bentley, W.E., and T.K. Wood, (2005) Regiospecific Oxidation of Naphthalene and Fluorene by Toluene Monooxygenase and Engineered Toluene 4-Monooxygenase of Pseudomonas mendocina KR1, Biotechnology and Bioengineering, 90:85-94.

Cha, H.J., Shin, H.S., Lim, H.J., Cho, H.S., Dalal, N.N., Pham, M.Q., and W.E. Bentley (2005) Comparative Production of Human Interleukin-2 Fused with Green Fluorescent Protein in Several Recombinant Expression Systems, Biochemical Engineering Journal, 24:225-233.

Lu C.H., Albano C.R., Bentley W.E., and G. Rao (2005) Quantitative and kinetic study of oxidative stress Regulons using green fluorescent protein, Biotechnology and Bioengineering, 89: 574-587.

Bleckwenn, N. A., Bentley, W. E., Shiloach, J., (2005) Vaccinia Virus-Based Expression of gp120 and EGFP: Survey of Mammalian Host Cell Lines, Biotechnology Progress, 21:186-191.

Bleckwenn, N. A., Bentley, W. E., Shiloach, J., (2005) Evaluation of Production Parameters with the Vaccinia Virus Expression System Using Microcarrier Attached HeLa Cells, Biotechnology Progress, 21:554-561.

Bleckwenn, N. A., Bentley, W. E., Shiloach, J., (2005) Production of Recombinant Protein Using the HeLa S3-Vaccinia Virus Expression System: Bioreactor Perfusion and Effects of Post-Infection Temperature, Journal of Bioscience, Biotechnology and Biochemistry, 69:1065-1072.

Bleckwenn, N. A., Golding, H., Bentley, W. E., Shiloach, J., (2005) Production of Recombinant Proteins by Vaccinia Virus in a Microcarrier Based Mammalian Cell Perfusion Bioreactor, Biotechnology and Bioengineering, 90:663-674.

Tao, Y., Bentley, W.E., and Wood, T.K. (2005) Phenol and 2-Naphthol Production by Toluene 4-Monooxygenases Using an Aqueous/Dioctyl Phthalate system, Applied Microbiology & Biotechnology, 68:614-621. 

Dalal, N.G., Bentley, W.E., and H.J. Cha (2005) Facile Monitoring of Baculovirus Infection for Foreign Protein Expression under Very Late Polyhedrin Promoter using Green Fluorescent Protein Reporter under Early-to-Late Promoter, Biochemical Engineering Journal, 24:27-30.

Cha, H.J., Dalal, N.G., and Bentley, W.E. (2005) Secretion of Human Interleukin-2 Fused with Green Fluorescent Protein in Recombinant Pichia pastoris, Applied Biochemistry and Biotechnology, 126:1-11.

Powers, M.A., S. T. Koev, A. Schleunitz, H. Yi, V. Hodzic, W. E. Bentley, G. F. Payne, G. W. Rubloff, and R. Ghodssi (2005) Integrated Optical Sensing of Biofunctionalized Sites in BioMEMS, Lab on a Chip, 5:583-586.

Chryssis, A.N., Saini, S.S., Lee, S.M., Yi, H.M., Bentley, W.E., and M. Dagenais (2005) Detecting Hybridization of DNA by Highly Selective Evanescent Field Etched Core Fiber Bragg Grating Sensors, IEEE Journal of Selected Topics in Quantum Electronics, 11:864-872.

Chang, W., Small, D.A. Toghrol, F., and W.E. Bentley (2005) Microarray Analysis of Toxicogenomic Effects of Peracetic Acid on Pseudomonas aeruginosa, Environmental Science & Technology, 39:5893-5899.

Chang, W., Small, D.A., Toghrol, F., and W.E. Bentley (2005) Microarray Analysis of Pseudomonas aeruginosa Reveals Induction of Pyocin Genes in Response to Hydrogen Peroxide, BMC Genomics, 6.115-129.

Yi, H., Nisar, S., Lee, S.Y., Powers, M.A., Bentley, W.E., Payne, GF, Ghodssi, R., Rubloff, G.W., Harris, M.T., Culver, J.N. (2005) Patterned Assembly of Genetically Modified Viral Nanotemplates via Nucleic Acid Hybridization, Nano Letters, 5:1031-1936.

Wang, L., Li, J., March, J.C., Valdes, J.J., and W.E. Bentley (2005), luxS-Dependent Gene Regulation in Escherichia coli K-12 Revealed by Genomic Expression Profiling, Journal of Bacteriology, 187:8350-8360.

Yi, H., Wu, L.Q., Bentley, W.E., Ghodssi, R., Rubloff, G.W., Culver, J.N., Payne, G.F., (2005) Biofabrication with Chitosan, Biomacromolecules, 6:2881-2894.

For more information see Dr. Bentley's other web pages:
The Biochip Collaborative »
Group Page: Molecular & Metabolic Engineering Laboratories »