Professor and Chair William Bentley
Chair
Robert E. Fischell Distinguished Professor
Joint appointments with UMBI and MTECH
Ph.D., University of Colorado at Boulder, 1989
Room 2330 Jeong H. Kim Engineering Building
Email: bentley@umd.edu
Phone: (301) 405-4321
Fax: (301) 405-9953
For more information please visit:
Message from the Chair »
The Biochip Collaborative »
Center for Biosystems Research »
Group Page: Molecular & Metabolic Engineering Laboratories »
William E. Bentley is the Robert E. Fischell Distinguished Professor and Chair of the Fischell Department of Bioengineering. He holds joint appointments with the Maryland Technology Enterprise Institute (MTECH; formerly the Engineering Research Center) and the University of Maryland Biotechnology Institute.
Dr. Bentley has an outstanding record of technical contributions in metabolic engineering, modeling of genetic circuits, cellular stress responses and E. coli protein expression, bioreactor design and optimization, and insect cell and Larvae/Baculovirus expression systems. His work is internationally recognized.
He received his B.S. and M.S. from Cornell University and his Ph.D. from the University of Colorado, Boulder, all in chemical engineering.
Biomolecular and metabolic engineering, cell-to-cell communication, heterologous protein expression, device/bio interfaces.
Top- Robert E. Fischell Distinguished Professor
- Herbert Rabin Distinguished Professor (former)
- Fellow, American Academy of Microbiology (AAM)
- Fellow, American Association for the Advancement of Science
- Fellow, American Institute for Medical and Biological Engineering
- Schering-Plough Young Investigator Award (1996)
- Dow Outstanding New Faculty Award from the American Society for Engineering Education (1995)
- Society of Industrial Microbiology Outstanding Achievement in Engineering Sciences Award
- Washington Academy of Sciences Allen C. Davis Medal
- Maryland's Outstanding Young Engineer, Maryland Science Center
Selected Recent Refereed Publications
Lee, J., Bansal, T., Jayaraman, A., Bentley, W.E., and T.K. Wood (2007) Enterohemorrhagic Escherichia coli Biofilms are Inhibited by 7-Hydroxyindole and Stimulated by Isatin, Applied and Environmental Microbiology.
Yung, C.W., Tullman, J.A., Payne, G.F., Bentley, W.E., and T.A. Barbari (2007) Transglutaminase Crosslinked Gelatin as a Tissue Engineering Scaffold, Journal of Biomedical Materials Research A, (Epub ahead of print).
Li, J., Attila, C., Wang, L., Wood, T.K., Valdes, J.J., and W.E. Bentley (2007) Quorum Sensing in E. coli is Signaled by AI-2/LsrR: Effects on sRNA and Biofilm Architecture, Journal of Bacteriology, 73:4100-4109.
Fernandes, R., Tsao, C.Y., Hashimoto, Y., Wang, L., Wood, T.K., Payne, G.F., and W.E. Bentley (2007) Magnetic Nanofactories: Localized Synthesis and Delivery of Quorum-Sensing Signaling Molecule Autoinducer-2 to Bacterial Cell Surfaces, Metabolic Engineering, 9:228-239.
Small, D.A., Chang, W., Bentley, W.E., and F. Toghrol (2007) Toxicogenomic Analysis of Sodium Hypochlorite Antimicrobial Mechanisms in Pseudomonas aeruginosa, Applied Microbiology and Biotechnology, 74:176-185.
Kim, E.J., Kramer, S.F., Hebert, C.G., Valdes, J.J., and W.E. Bentley (2007) Metabolic Engineering of the Baculovirus-Expression System via Inverse "Shotgun" Genomic Analysis & RNA Interference (dsRNA) Increases Product Yield and Cell Longevity, Biotechnology and Bioengineering, 98:645-654.
March, J.C., and W.E. Bentley (2007) RNAi-based Tuning of Cell Cycling in Drosophila S2 cells- effects on Recombinant Protein Yield, Applied Microbiology and Biotechnology, 73: 1128-1135.
Koev, S.T., Powers, M. A. Powers, Yi, H.M., Wu, L-Q., Bentley, W.E., Rubloff, G.W., Payne, G.F., and R. Ghodssi, (2007) Mechano-transduction of DNA Hybridization and Dopamine Oxidation through Electrodeposited Chitosan Network, Lab on a Chip, 7:103-111.
Small, D.A, Chang, W., Toghrol, F., and W.E. Bentley (2007) Comparative Global Transcription Analysis of Sodium Hypochlorite, Peracetic Acid, and Hydrogen Peroxide on Pseudomonas aeruginosa, Applied Microbiology and Biotechnology, 76:1093-1105.
O’Connell, K.P., Kovaleva, E., Campbell, J.H., Anderson, P.E., Brown, S.G., Davis, D.C., Valdes, J.J., Welch, R.W., Bentley, W.E. and N.A. van Beek (2007) Production of a Recombinant Antibody Fragment in Whole Insect Larvae, Molecular Biotechnology, 36:44-51.
Hung, C-W., Holoman, T.R.P., Kofinas, P., and W.E. Bentley (2007) Towards Oriented Assembly of Proteins onto Magnetic Nanoparticles, Biochemical Engineering Journal.
Saini, S.S., Stanford, C., Lee, S.M., Park, J., DeShong, P., Bentley, W.E., and M. Dagenais (2007) Monolayer Detection of Biochemical Agents Using Etched-Core Fiber Bragg Grating Sensors, IEEE Photonics Technology Letters, 19:1341-1343.
Lewandowski, A.T., Yi, H., Luo, X., Payne, G.F., Ghodssi, R., Rubloff, G.W., and W.E. Bentley (2007) Protein Assembly onto Patterned Microfabricated Devices through Enzymatic Activation of Fusion Pro-tag. Biotechnology and Bioengineering.
March, J.C., and W.E. Bentley (2007) Methods for gene silencing with RNAi, Methods in Molecular Biology, 388:424-434.
Hu, Y.C., and W.E. Bentley (2007) Alternative Bioreactor Strategy for Probing Infection and Production, Methods in Molecular Biology, 388:419-426.
Cha, H.J., and W.E. Bentley (2007) Monitoring and Visualization of Baculovirus Infection Using Green Fluorescent Protein Strategy, Methods in Molecular Biology, 388:407-418.
Lewandowski, A.T., Small, D.A., Chen, T., Payne, G.F., and W.E. Bentley (2006) Tyrosine-based "activatable pro-tag": enzyme-catalyzed protein capture and release, Biotechnology and Bioengineering, 93:1207-1215.
Chang, W., Small, D.A., Toghrol, F., and W.E. Bentley (2006) Global Transcriptome Analysis of Staphylococcus aureus Response to Hydrogen Peroxide, Journal of Bacteriology, 188:1648-1659.
Gonzalez Barrios, A.F., Zuo R., Hashimoto, Y., Yang, L., Bentley W.E., and T.K. Wood (2006) Autoinducer 2 Controls Biofilm Formation in Escherichia coli Through a Novel Motility Quorum-sensing Regulator (MqsR, B3022), Journal of Bacteriology, 188:205-316.
Bleckwenn, N. A. and Bentley, W.E. (2006) Systems and Technology Involving Bacteria, In CRC BME Handbook: Tissue Engineering and Artificial Organs (JD Bronzino, ed.) CRC Press, Boca Raton, FL, 2.1-2.10.
Yung, C., Barbari, T.A., and W.E. Bentley (2006) Integrated Non-Invasive System for Quantifying Secreted Human Therapeutic hIL2, Biotechnology and Bioengineering.
Yang, L., Portugal, F.H., and W.E. Bentley (2006) Conditioned Medium from Listeria innocua Stimulate Emergence from a Resting State not in Response to Quorum Sensing Autoinducer AI-2, Biotechnology Progress, 22:387-393.
March, J.C., and W.E. Bentley (2006) Engineering Eukaryotic Signal Transduction with RNAi: Enhancing Drosophila S2 Cell Growth and Recombinant Protein Synthesis via Silencing of TSC1. Biotechnology and Bioengineering.
Park, J.J., Valentine, T.M., Yi, H., Payne, G.F., Bentley, W.E., Ghodssi, R., and Gary W. Rubloff (2006) In situ Biomolecule Assembly in Completely Packaged Microfluidic Devices, Lab on a Chip.
Yung, C.W., Barbari, T.A., and W.E. Bentley (2006) Counteracting Apoptosis and Necrosis with Hypoxia Responsive Expression of Bcl-2D, Metabolic Engineering.
Cresce, A.V., Silverstein, J.S., Bentley, W.E., and P. Kofinas (2006) Nanopatterning of Recombinant Proteins using Block Copolymer Templates, Macromolecules, 39, 5826-5829.
Dalal, N.G., Cha, H.J., Kramer, S.F., Kostov, Y., Rao, G. and W.E. Bentley (2006) Rapid Non-invasive Monitoring of Baculovirus Infection for Insect Larvae Using Green Fluorescent Protein Reporter Under Early-to-Late Promoter and a GFP-Specific Optical Probe, Process Biochemistry, 41:947-950.
Li, J., Wang, L., Hashimoto, Y., Tsao, C-Y., Wood, T.K., Valdes, J.J., Zafiriou, E., and W.E. Bentley (2006) Stochastic Model of E. coli AI-2 Quorum Signal Circuit Reveals Alternative Synthesis Pathways, Nature Molecular Systems Biology.
Wang, L., Hashimoto, Y., Tsao, C.-Y., Valdes, J.J., and W.E. Bentley (2005) cAMP and cAMP Receptor Protein (CRP) Influence Both Synthesis and Uptake of Extracellular Autoinducer-2 in Escherichia coli, Journal of Bacteriology, 187:2066-76.
Yi, H., Wu, L.Q., Rubloff, G.W., Ghodssi, R., Payne, G.F., and Bentley, W.E., (2005) Signal-Directed Sequential Assembly of Biomolecules on Patterned Surfaces. Langmuir, 2:2104-2107.
Tao, Y., Bentley, W.E., and T.K. Wood, (2005) Regiospecific Oxidation of Naphthalene and Fluorene by Toluene Monooxygenase and Engineered Toluene 4-Monooxygenase of Pseudomonas mendocina KR1, Biotechnology and Bioengineering, 90:85-94.
Cha, H.J., Shin, H.S., Lim, H.J., Cho, H.S., Dalal, N.N., Pham, M.Q., and W.E. Bentley (2005) Comparative Production of Human Interleukin-2 Fused with Green Fluorescent Protein in Several Recombinant Expression Systems, Biochemical Engineering Journal, 24:225-233.
Lu C.H., Albano C.R., Bentley W.E., and G. Rao (2005) Quantitative and kinetic study of oxidative stress Regulons using green fluorescent protein, Biotechnology and Bioengineering, 89: 574-587.
Bleckwenn, N. A., Bentley, W. E., Shiloach, J., (2005) Vaccinia Virus-Based Expression of gp120 and EGFP: Survey of Mammalian Host Cell Lines, Biotechnology Progress, 21:186-191.
Bleckwenn, N. A., Bentley, W. E., Shiloach, J., (2005) Evaluation of Production Parameters with the Vaccinia Virus Expression System Using Microcarrier Attached HeLa Cells, Biotechnology Progress, 21:554-561.
Bleckwenn, N. A., Bentley, W. E., Shiloach, J., (2005) Production of Recombinant Protein Using the HeLa S3-Vaccinia Virus Expression System: Bioreactor Perfusion and Effects of Post-Infection Temperature, Journal of Bioscience, Biotechnology and Biochemistry, 69:1065-1072.
Bleckwenn, N. A., Golding, H., Bentley, W. E., Shiloach, J., (2005) Production of Recombinant Proteins by Vaccinia Virus in a Microcarrier Based Mammalian Cell Perfusion Bioreactor, Biotechnology and Bioengineering, 90:663-674.
Tao, Y., Bentley, W.E., and Wood, T.K. (2005) Phenol and 2-Naphthol Production by Toluene 4-Monooxygenases Using an Aqueous/Dioctyl Phthalate system, Applied Microbiology & Biotechnology, 68:614-621.
Dalal, N.G., Bentley, W.E., and H.J. Cha (2005) Facile Monitoring of Baculovirus Infection for Foreign Protein Expression under Very Late Polyhedrin Promoter using Green Fluorescent Protein Reporter under Early-to-Late Promoter, Biochemical Engineering Journal, 24:27-30.
Cha, H.J., Dalal, N.G., and Bentley, W.E. (2005) Secretion of Human Interleukin-2 Fused with Green Fluorescent Protein in Recombinant Pichia pastoris, Applied Biochemistry and Biotechnology, 126:1-11.
Powers, M.A., S. T. Koev, A. Schleunitz, H. Yi, V. Hodzic, W. E. Bentley, G. F. Payne, G. W. Rubloff, and R. Ghodssi (2005) Integrated Optical Sensing of Biofunctionalized Sites in BioMEMS, Lab on a Chip, 5:583-586.
Chryssis, A.N., Saini, S.S., Lee, S.M., Yi, H.M., Bentley, W.E., and M. Dagenais (2005) Detecting Hybridization of DNA by Highly Selective Evanescent Field Etched Core Fiber Bragg Grating Sensors, IEEE Journal of Selected Topics in Quantum Electronics, 11:864-872.
Chang, W., Small, D.A. Toghrol, F., and W.E. Bentley (2005) Microarray Analysis of Toxicogenomic Effects of Peracetic Acid on Pseudomonas aeruginosa, Environmental Science & Technology, 39:5893-5899.
Chang, W., Small, D.A., Toghrol, F., and W.E. Bentley (2005) Microarray Analysis of Pseudomonas aeruginosa Reveals Induction of Pyocin Genes in Response to Hydrogen Peroxide, BMC Genomics, 6.115-129.
Yi, H., Nisar, S., Lee, S.Y., Powers, M.A., Bentley, W.E., Payne, GF, Ghodssi, R., Rubloff, G.W., Harris, M.T., Culver, J.N. (2005) Patterned Assembly of Genetically Modified Viral Nanotemplates via Nucleic Acid Hybridization, Nano Letters, 5:1031-1936.
Wang, L., Li, J., March, J.C., Valdes, J.J., and W.E. Bentley (2005), luxS-Dependent Gene Regulation in Escherichia coli K-12 Revealed by Genomic Expression Profiling, Journal of Bacteriology, 187:8350-8360.
Yi, H., Wu, L.Q., Bentley, W.E., Ghodssi, R., Rubloff, G.W., Culver, J.N., Payne, G.F., (2005) Biofabrication with Chitosan, Biomacromolecules, 6:2881-2894.
For more information see Dr. Bentley's
other web pages:
Center for Biosystems Research
Group Page: Molecular and Metabolic Engineering Laboratories
